This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -q 20 -m 36 -O 3 -o fastq/zr1394_11_s456.fastq.gz zr1394_11_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1372.94 s (25 us/read; 2.41 M reads/minute). === Summary === Total reads processed: 55,109,528 Reads with adapters: 284,799 (0.5%) Reads written (passing filters): 53,656,597 (97.4%) Total basepairs processed: 2,769,826,173 bp Quality-trimmed: 3,004,243 bp (0.1%) Total written (filtered): 2,173,152,997 bp (78.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 284799 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.4% C: 2.0% G: 33.7% T: 37.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 226137 861086.4 0 226137 4 45750 215271.6 0 45750 5 7216 53817.9 0 7216 6 682 13454.5 0 682 7 1872 3363.6 0 1872 8 730 840.9 0 730 9 346 210.2 0 39 307 10 807 52.6 1 7 800 11 265 13.1 1 28 237 12 146 3.3 1 0 146 13 58 0.8 1 0 58 14 44 0.8 1 0 44 15 66 0.8 1 0 66 16 170 0.8 1 0 170 17 309 0.8 1 0 309 18 168 0.8 1 0 168 19 20 0.8 1 0 20 20 2 0.8 1 0 2 21 3 0.8 1 0 3 22 1 0.8 1 0 1 23 2 0.8 1 0 2 24 1 0.8 1 0 1 25 2 0.8 1 0 2 27 1 0.8 1 0 1 28 1 0.8 1 0 1