This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -q 20 -m 36 -O 3 -o fastq/zr1394_10_s456.fastq.gz zr1394_10_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1896.41 s (24 us/read; 2.51 M reads/minute). === Summary === Total reads processed: 79,264,583 Reads with adapters: 521,180 (0.7%) Reads written (passing filters): 76,863,174 (97.0%) Total basepairs processed: 3,978,999,570 bp Quality-trimmed: 5,069,089 bp (0.1%) Total written (filtered): 3,110,263,584 bp (78.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 521180 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.8% C: 1.4% G: 34.5% T: 37.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 417921 1238509.1 0 417921 4 81597 309627.3 0 81597 5 12135 77406.8 0 12135 6 1254 19351.7 0 1254 7 4143 4837.9 0 4143 8 1302 1209.5 0 1302 9 670 302.4 0 45 625 10 1591 75.6 1 5 1586 11 252 18.9 1 16 236 12 147 4.7 1 0 147 13 34 1.2 1 0 34 14 25 1.2 1 0 25 15 18 1.2 1 0 18 16 15 1.2 1 0 15 17 47 1.2 1 0 47 18 19 1.2 1 0 19 19 5 1.2 1 0 5 20 3 1.2 1 0 3 23 1 1.2 1 0 1 25 1 1.2 1 0 1