This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_9_s456.fastq.gz zr1394_9_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 2061.71 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 78,146,508 Reads with adapters: 413,331 (0.5%) Reads written (passing filters): 77,712,105 (99.4%) Total basepairs processed: 3,934,245,065 bp Quality-trimmed: 3,699,261 bp (0.1%) Total written (filtered): 3,916,862,007 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 413331 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.8% C: 1.4% G: 33.9% T: 37.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 325643 1221039.2 0 325643 4 73128 305259.8 0 73128 5 8030 76314.9 0 8030 6 742 19078.7 0 742 7 2218 4769.7 0 2218 8 800 1192.4 0 800 9 473 298.1 0 40 433 10 852 74.5 1 8 844 11 285 18.6 1 24 261 12 140 4.7 1 0 140 13 50 1.2 1 0 50 14 51 1.2 1 0 51 15 69 1.2 1 0 69 16 154 1.2 1 0 154 17 392 1.2 1 0 392 18 245 1.2 1 0 245 19 20 1.2 1 0 20 20 4 1.2 1 0 4 21 2 1.2 1 0 2 22 2 1.2 1 0 2 23 1 1.2 1 0 1 24 5 1.2 1 0 5 25 3 1.2 1 0 3 26 3 1.2 1 0 3 27 6 1.2 1 0 6 28 5 1.2 1 0 5 29 2 1.2 1 0 2 31 1 1.2 1 0 1 32 2 1.2 1 0 2 33 2 1.2 1 0 2 34 1 1.2 1 0 1