This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_8_s456.fastq.gz zr1394_8_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1094.43 s (28 us/read; 2.15 M reads/minute). === Summary === Total reads processed: 39,136,514 Reads with adapters: 230,842 (0.6%) Reads written (passing filters): 38,891,479 (99.4%) Total basepairs processed: 1,968,545,164 bp Quality-trimmed: 2,012,847 bp (0.1%) Total written (filtered): 1,958,829,733 bp (99.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 230842 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.2% C: 1.7% G: 36.7% T: 35.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 184704 611508.0 0 184704 4 36406 152877.0 0 36406 5 4814 38219.3 0 4814 6 470 9554.8 0 470 7 1447 2388.7 0 1447 8 486 597.2 0 486 9 271 149.3 0 21 250 10 614 37.3 1 13 601 11 250 9.3 1 26 224 12 135 2.3 1 0 135 13 62 0.6 1 0 62 14 50 0.6 1 0 50 15 78 0.6 1 0 78 16 178 0.6 1 0 178 17 460 0.6 1 0 460 18 320 0.6 1 0 320 19 42 0.6 1 0 42 20 4 0.6 1 0 4 21 5 0.6 1 0 5 22 4 0.6 1 0 4 23 3 0.6 1 0 3 24 3 0.6 1 0 3 25 7 0.6 1 0 7 26 3 0.6 1 0 3 27 6 0.6 1 0 6 28 7 0.6 1 0 7 29 2 0.6 1 0 2 30 1 0.6 1 0 1 31 1 0.6 1 0 1 32 2 0.6 1 0 2 33 3 0.6 1 0 3 34 2 0.6 1 0 2 35 1 0.6 1 0 1 37 1 0.6 1 0 1