This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_7_s456.fastq.gz zr1394_7_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1415.16 s (28 us/read; 2.14 M reads/minute). === Summary === Total reads processed: 50,399,353 Reads with adapters: 298,147 (0.6%) Reads written (passing filters): 50,029,410 (99.3%) Total basepairs processed: 2,532,578,583 bp Quality-trimmed: 2,675,889 bp (0.1%) Total written (filtered): 2,518,377,809 bp (99.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 298147 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.8% C: 2.5% G: 32.8% T: 36.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 233073 787489.9 0 233073 4 47951 196872.5 0 47951 5 6971 49218.1 0 6971 6 678 12304.5 0 678 7 1959 3076.1 0 1959 8 1002 769.0 0 1002 9 471 192.3 0 58 413 10 855 48.1 1 19 836 11 465 12.0 1 57 408 12 302 3.0 1 4 298 13 133 0.8 1 0 133 14 127 0.8 1 0 127 15 216 0.8 1 0 216 16 561 0.8 1 0 561 17 1592 0.8 1 0 1592 18 1378 0.8 1 0 1378 19 143 0.8 1 0 143 20 22 0.8 1 0 22 21 16 0.8 1 0 16 22 6 0.8 1 0 6 23 12 0.8 1 0 12 24 16 0.8 1 0 16 25 15 0.8 1 0 15 26 33 0.8 1 0 33 27 32 0.8 1 0 32 28 21 0.8 1 0 21 29 5 0.8 1 0 5 30 10 0.8 1 0 10 31 18 0.8 1 0 18 32 23 0.8 1 0 23 33 15 0.8 1 0 15 34 15 0.8 1 0 15 35 7 0.8 1 0 7 36 2 0.8 1 0 2 37 2 0.8 1 0 2