This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_6_s456.fastq.gz zr1394_6_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1312.49 s (28 us/read; 2.15 M reads/minute). === Summary === Total reads processed: 46,944,411 Reads with adapters: 263,186 (0.6%) Reads written (passing filters): 46,663,251 (99.4%) Total basepairs processed: 2,363,416,290 bp Quality-trimmed: 2,138,751 bp (0.1%) Total written (filtered): 2,352,467,999 bp (99.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 263186 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.3% C: 1.7% G: 34.5% T: 37.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 205047 733506.4 0 205047 4 46939 183376.6 0 46939 5 4257 45844.2 0 4257 6 546 11461.0 0 546 7 1292 2865.3 0 1292 8 569 716.3 0 569 9 311 179.1 0 31 280 10 633 44.8 1 10 623 11 287 11.2 1 35 252 12 205 2.8 1 1 204 13 93 0.7 1 0 93 14 89 0.7 1 0 89 15 163 0.7 1 0 163 16 366 0.7 1 0 366 17 1103 0.7 1 0 1103 18 992 0.7 1 0 992 19 108 0.7 1 0 108 20 16 0.7 1 0 16 21 11 0.7 1 0 11 22 7 0.7 1 0 7 23 9 0.7 1 0 9 24 12 0.7 1 0 12 25 16 0.7 1 0 16 26 23 0.7 1 0 23 27 13 0.7 1 0 13 28 8 0.7 1 0 8 29 6 0.7 1 0 6 30 14 0.7 1 0 14 31 8 0.7 1 0 8 32 13 0.7 1 0 13 33 13 0.7 1 0 13 34 12 0.7 1 0 12 35 4 0.7 1 0 4 39 1 0.7 1 0 1