This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_5_s456.fastq.gz zr1394_5_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1348.21 s (28 us/read; 2.14 M reads/minute). === Summary === Total reads processed: 48,050,056 Reads with adapters: 320,748 (0.7%) Reads written (passing filters): 47,694,564 (99.3%) Total basepairs processed: 2,414,803,052 bp Quality-trimmed: 2,459,373 bp (0.1%) Total written (filtered): 2,401,111,591 bp (99.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 320748 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.0% C: 2.1% G: 35.4% T: 35.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 253524 750782.1 0 253524 4 51698 187695.5 0 51698 5 6400 46923.9 0 6400 6 650 11731.0 0 650 7 1757 2932.7 0 1757 8 1013 733.2 0 1013 9 476 183.3 0 60 416 10 803 45.8 1 16 787 11 402 11.5 1 50 352 12 289 2.9 1 1 288 13 129 0.7 1 0 129 14 140 0.7 1 0 140 15 231 0.7 1 0 231 16 572 0.7 1 0 572 17 1330 0.7 1 0 1330 18 1058 0.7 1 0 1058 19 104 0.7 1 0 104 20 22 0.7 1 0 22 21 10 0.7 1 0 10 22 10 0.7 1 0 10 23 11 0.7 1 0 11 24 10 0.7 1 0 10 25 9 0.7 1 0 9 26 17 0.7 1 0 17 27 18 0.7 1 0 18 28 11 0.7 1 0 11 29 8 0.7 1 0 8 30 11 0.7 1 0 11 31 7 0.7 1 0 7 32 4 0.7 1 0 4 33 7 0.7 1 0 7 34 10 0.7 1 0 10 35 5 0.7 1 0 5 36 2 0.7 1 0 2