This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_4_s456.fastq.gz zr1394_4_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1274.44 s (28 us/read; 2.14 M reads/minute). === Summary === Total reads processed: 45,411,925 Reads with adapters: 260,318 (0.6%) Reads written (passing filters): 45,124,223 (99.4%) Total basepairs processed: 2,284,287,588 bp Quality-trimmed: 2,381,487 bp (0.1%) Total written (filtered): 2,272,951,821 bp (99.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 260318 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 25.9% C: 1.7% G: 36.4% T: 36.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 205565 709561.3 0 205565 4 42473 177390.3 0 42473 5 5508 44347.6 0 5508 6 614 11086.9 0 614 7 1579 2771.7 0 1579 8 658 692.9 0 658 9 360 173.2 0 34 326 10 696 43.3 1 11 685 11 290 10.8 1 42 248 12 178 2.7 1 3 175 13 87 0.7 1 0 87 14 73 0.7 1 0 73 15 140 0.7 1 0 140 16 323 0.7 1 0 323 17 895 0.7 1 0 895 18 680 0.7 1 0 680 19 79 0.7 1 0 79 20 14 0.7 1 0 14 21 12 0.7 1 0 12 22 9 0.7 1 0 9 23 4 0.7 1 0 4 24 7 0.7 1 0 7 25 9 0.7 1 0 9 26 18 0.7 1 0 18 27 7 0.7 1 0 7 28 6 0.7 1 0 6 29 5 0.7 1 0 5 30 5 0.7 1 0 5 31 4 0.7 1 0 4 32 1 0.7 1 0 1 33 8 0.7 1 0 8 34 8 0.7 1 0 8 35 1 0.7 1 0 1 36 1 0.7 1 0 1 37 1 0.7 1 0 1