This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_3_s456.fastq.gz zr1394_3_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1370.83 s (28 us/read; 2.14 M reads/minute). === Summary === Total reads processed: 48,926,620 Reads with adapters: 278,010 (0.6%) Reads written (passing filters): 48,615,676 (99.4%) Total basepairs processed: 2,461,374,827 bp Quality-trimmed: 2,449,172 bp (0.1%) Total written (filtered): 2,449,194,143 bp (99.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 278010 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 25.8% C: 1.8% G: 36.1% T: 36.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 219964 764478.4 0 219964 4 45745 191119.6 0 45745 5 5398 47779.9 0 5398 6 518 11945.0 0 518 7 1564 2986.2 0 1564 8 655 746.6 0 655 9 292 186.6 0 30 262 10 785 46.7 1 17 768 11 299 11.7 1 20 279 12 194 2.9 1 2 192 13 108 0.7 1 0 108 14 60 0.7 1 0 60 15 155 0.7 1 0 155 16 357 0.7 1 0 357 17 945 0.7 1 0 945 18 761 0.7 1 0 761 19 71 0.7 1 0 71 20 18 0.7 1 0 18 21 7 0.7 1 0 7 22 3 0.7 1 0 3 23 6 0.7 1 0 6 24 5 0.7 1 0 5 25 9 0.7 1 0 9 26 12 0.7 1 0 12 27 10 0.7 1 0 10 28 13 0.7 1 0 13 29 6 0.7 1 0 6 30 8 0.7 1 0 8 31 10 0.7 1 0 10 32 8 0.7 1 0 8 33 5 0.7 1 0 5 34 10 0.7 1 0 10 35 3 0.7 1 0 3 36 2 0.7 1 0 2 37 2 0.7 1 0 2 38 2 0.7 1 0 2