This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_2_s456.fastq.gz zr1394_2_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1313.13 s (28 us/read; 2.13 M reads/minute). === Summary === Total reads processed: 46,666,438 Reads with adapters: 293,867 (0.6%) Reads written (passing filters): 46,350,669 (99.3%) Total basepairs processed: 2,346,424,714 bp Quality-trimmed: 2,397,497 bp (0.1%) Total written (filtered): 2,334,082,648 bp (99.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 293867 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.3% C: 1.9% G: 36.0% T: 35.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 231867 729163.1 0 231867 4 47968 182290.8 0 47968 5 5845 45572.7 0 5845 6 652 11393.2 0 652 7 1874 2848.3 0 1874 8 729 712.1 0 729 9 427 178.0 0 33 394 10 865 44.5 1 9 856 11 376 11.1 1 57 319 12 209 2.8 1 0 209 13 100 0.7 1 0 100 14 99 0.7 1 0 99 15 182 0.7 1 0 182 16 439 0.7 1 0 439 17 1067 0.7 1 0 1067 18 932 0.7 1 0 932 19 83 0.7 1 0 83 20 22 0.7 1 0 22 21 7 0.7 1 0 7 22 14 0.7 1 0 14 23 6 0.7 1 0 6 24 6 0.7 1 0 6 25 13 0.7 1 0 13 26 16 0.7 1 0 16 27 8 0.7 1 0 8 28 8 0.7 1 0 8 29 13 0.7 1 0 13 30 3 0.7 1 0 3 31 8 0.7 1 0 8 32 12 0.7 1 0 12 33 7 0.7 1 0 7 34 6 0.7 1 0 6 35 1 0.7 1 0 1 36 1 0.7 1 0 1 37 1 0.7 1 0 1 39 1 0.7 1 0 1