This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_1_s456.fastq.gz zr1394_1_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1324.58 s (28 us/read; 2.15 M reads/minute). === Summary === Total reads processed: 47,399,413 Reads with adapters: 289,331 (0.6%) Reads written (passing filters): 47,061,637 (99.3%) Total basepairs processed: 2,382,626,805 bp Quality-trimmed: 2,279,442 bp (0.1%) Total written (filtered): 2,369,734,766 bp (99.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 289331 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.1% C: 2.4% G: 34.9% T: 35.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 227505 740615.8 0 227505 4 45947 185154.0 0 45947 5 6341 46288.5 0 6341 6 636 11572.1 0 636 7 1722 2893.0 0 1722 8 731 723.3 0 731 9 358 180.8 0 43 315 10 835 45.2 1 19 816 11 429 11.3 1 46 383 12 264 2.8 1 4 260 13 130 0.7 1 0 130 14 131 0.7 1 0 131 15 205 0.7 1 0 205 16 539 0.7 1 0 539 17 1620 0.7 1 0 1620 18 1478 0.7 1 0 1478 19 166 0.7 1 0 166 20 33 0.7 1 0 33 21 15 0.7 1 0 15 22 7 0.7 1 0 7 23 14 0.7 1 0 14 24 13 0.7 1 0 13 25 14 0.7 1 0 14 26 38 0.7 1 0 38 27 34 0.7 1 0 34 28 17 0.7 1 0 17 29 12 0.7 1 0 12 30 11 0.7 1 0 11 31 21 0.7 1 0 21 32 15 0.7 1 0 15 33 15 0.7 1 0 15 34 19 0.7 1 0 19 35 11 0.7 1 0 11 36 3 0.7 1 0 3 37 1 0.7 1 0 1 38 1 0.7 1 0 1