This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_18_s456.fastq.gz zr1394_18_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1548.90 s (28 us/read; 2.15 M reads/minute). === Summary === Total reads processed: 55,559,681 Reads with adapters: 310,611 (0.6%) Reads written (passing filters): 55,278,688 (99.5%) Total basepairs processed: 2,797,597,463 bp Quality-trimmed: 2,697,516 bp (0.1%) Total written (filtered): 2,785,964,289 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 310611 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.3% C: 1.5% G: 34.4% T: 36.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 244932 868120.0 0 244932 4 53822 217030.0 0 53822 5 6117 54257.5 0 6117 6 628 13564.4 0 628 7 1872 3391.1 0 1872 8 655 847.8 0 655 9 371 211.9 0 27 344 10 865 53.0 1 10 855 11 285 13.2 1 31 254 12 142 3.3 1 1 141 13 71 0.8 1 0 71 14 64 0.8 1 0 64 15 76 0.8 1 0 76 16 170 0.8 1 0 170 17 266 0.8 1 0 266 18 201 0.8 1 0 201 19 26 0.8 1 0 26 20 6 0.8 1 0 6 21 4 0.8 1 0 4 22 3 0.8 1 0 3 23 4 0.8 1 0 4 24 6 0.8 1 0 6 25 3 0.8 1 0 3 26 9 0.8 1 0 9 27 2 0.8 1 0 2 28 1 0.8 1 0 1 29 4 0.8 1 0 4 31 2 0.8 1 0 2 32 1 0.8 1 0 1 33 1 0.8 1 0 1 35 2 0.8 1 0 2