This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_17_s456.fastq.gz zr1394_17_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1217.55 s (28 us/read; 2.11 M reads/minute). === Summary === Total reads processed: 42,730,485 Reads with adapters: 288,852 (0.7%) Reads written (passing filters): 42,384,963 (99.2%) Total basepairs processed: 2,143,526,203 bp Quality-trimmed: 2,502,691 bp (0.1%) Total written (filtered): 2,130,108,233 bp (99.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 288852 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 29.3% C: 2.4% G: 34.5% T: 33.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 232097 667663.8 0 232097 4 41330 166916.0 0 41330 5 7514 41729.0 0 7514 6 690 10432.2 0 690 7 1822 2608.1 0 1822 8 1037 652.0 0 1037 9 422 163.0 0 61 361 10 920 40.8 1 8 912 11 430 10.2 1 86 344 12 213 2.5 1 0 213 13 151 0.6 1 0 151 14 137 0.6 1 0 137 15 184 0.6 1 0 184 16 404 0.6 1 0 404 17 816 0.6 1 0 816 18 502 0.6 1 0 502 19 62 0.6 1 0 62 20 18 0.6 1 0 18 21 3 0.6 1 0 3 22 13 0.6 1 0 13 23 11 0.6 1 0 11 24 6 0.6 1 0 6 25 7 0.6 1 0 7 26 12 0.6 1 0 12 27 15 0.6 1 0 15 28 4 0.6 1 0 4 29 4 0.6 1 0 4 30 4 0.6 1 0 4 31 3 0.6 1 0 3 32 3 0.6 1 0 3 33 4 0.6 1 0 4 34 6 0.6 1 0 6 35 5 0.6 1 0 5 36 1 0.6 1 0 1 37 2 0.6 1 0 2