This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_16_s456.fastq.gz zr1394_16_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 2014.26 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 75,450,315 Reads with adapters: 403,048 (0.5%) Reads written (passing filters): 74,948,204 (99.3%) Total basepairs processed: 3,791,761,278 bp Quality-trimmed: 4,327,306 bp (0.1%) Total written (filtered): 3,771,850,595 bp (99.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 403048 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.8% C: 2.0% G: 33.0% T: 37.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 317779 1178911.2 0 317779 4 66050 294727.8 0 66050 5 10402 73681.9 0 10402 6 960 18420.5 0 960 7 2933 4605.1 0 2933 8 1094 1151.3 0 1094 9 602 287.8 0 66 536 10 1198 72.0 1 9 1189 11 374 18.0 1 39 335 12 182 4.5 1 1 181 13 69 1.1 1 0 69 14 71 1.1 1 0 71 15 90 1.1 1 0 90 16 235 1.1 1 0 235 17 515 1.1 1 0 515 18 381 1.1 1 0 381 19 42 1.1 1 0 42 20 12 1.1 1 0 12 21 4 1.1 1 0 4 22 4 1.1 1 0 4 23 1 1.1 1 0 1 24 5 1.1 1 0 5 25 4 1.1 1 0 4 26 3 1.1 1 0 3 27 13 1.1 1 0 13 28 2 1.1 1 0 2 29 9 1.1 1 0 9 30 2 1.1 1 0 2 31 4 1.1 1 0 4 32 4 1.1 1 0 4 33 1 1.1 1 0 1 34 1 1.1 1 0 1 35 1 1.1 1 0 1 36 1 1.1 1 0 1