This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_15_s456.fastq.gz zr1394_15_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1884.25 s (27 us/read; 2.19 M reads/minute). === Summary === Total reads processed: 68,857,992 Reads with adapters: 385,091 (0.6%) Reads written (passing filters): 68,375,877 (99.3%) Total basepairs processed: 3,459,545,810 bp Quality-trimmed: 4,066,729 bp (0.1%) Total written (filtered): 3,440,600,602 bp (99.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 385091 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.4% C: 2.1% G: 33.7% T: 36.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 304450 1075906.1 0 304450 4 62669 268976.5 0 62669 5 9679 67244.1 0 9679 6 889 16811.0 0 889 7 2500 4202.8 0 2500 8 1116 1050.7 0 1116 9 540 262.7 0 51 489 10 1303 65.7 1 15 1288 11 391 16.4 1 46 345 12 184 4.1 1 1 183 13 70 1.0 1 0 70 14 75 1.0 1 0 75 15 96 1.0 1 0 96 16 221 1.0 1 0 221 17 442 1.0 1 0 442 18 360 1.0 1 0 360 19 38 1.0 1 0 38 20 11 1.0 1 0 11 21 8 1.0 1 0 8 22 8 1.0 1 0 8 23 6 1.0 1 0 6 24 2 1.0 1 0 2 25 5 1.0 1 0 5 26 4 1.0 1 0 4 27 9 1.0 1 0 9 28 1 1.0 1 0 1 29 2 1.0 1 0 2 30 2 1.0 1 0 2 31 2 1.0 1 0 2 32 3 1.0 1 0 3 33 1 1.0 1 0 1 34 1 1.0 1 0 1 35 2 1.0 1 0 2 36 1 1.0 1 0 1