This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_13_s456.fastq.gz zr1394_13_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1781.19 s (27 us/read; 2.19 M reads/minute). === Summary === Total reads processed: 65,081,682 Reads with adapters: 354,786 (0.5%) Reads written (passing filters): 64,685,165 (99.4%) Total basepairs processed: 3,272,837,182 bp Quality-trimmed: 3,459,477 bp (0.1%) Total written (filtered): 3,256,971,770 bp (99.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 354786 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.5% C: 1.7% G: 34.3% T: 36.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 280330 1016901.3 0 280330 4 58268 254225.3 0 58268 5 8366 63556.3 0 8366 6 798 15889.1 0 798 7 2561 3972.3 0 2561 8 1035 993.1 0 1035 9 508 248.3 0 36 472 10 1126 62.1 1 2 1124 11 347 15.5 1 36 311 12 174 3.9 1 1 173 13 64 1.0 1 0 64 14 56 1.0 1 0 56 15 81 1.0 1 0 81 16 200 1.0 1 0 200 17 428 1.0 1 0 428 18 365 1.0 1 0 365 19 29 1.0 1 0 29 20 6 1.0 1 0 6 21 1 1.0 1 0 1 22 1 1.0 1 0 1 23 6 1.0 1 0 6 24 6 1.0 1 0 6 25 3 1.0 1 0 3 26 5 1.0 1 0 5 27 6 1.0 1 0 6 28 1 1.0 1 0 1 29 2 1.0 1 0 2 30 2 1.0 1 0 2 31 1 1.0 1 0 1 32 4 1.0 1 0 4 33 1 1.0 1 0 1 34 2 1.0 1 0 2 35 1 1.0 1 0 1 36 2 1.0 1 0 2