This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_12_s456.fastq.gz zr1394_12_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1653.60 s (27 us/read; 2.18 M reads/minute). === Summary === Total reads processed: 60,153,246 Reads with adapters: 336,700 (0.6%) Reads written (passing filters): 59,768,740 (99.4%) Total basepairs processed: 3,025,392,455 bp Quality-trimmed: 3,405,348 bp (0.1%) Total written (filtered): 3,010,096,869 bp (99.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 336700 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.7% C: 1.4% G: 32.6% T: 38.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 262540 939894.5 0 262540 4 61274 234973.6 0 61274 5 6867 58743.4 0 6867 6 674 14685.9 0 674 7 2226 3671.5 0 2226 8 854 917.9 0 854 9 404 229.5 0 34 370 10 865 57.4 1 10 855 11 211 14.3 1 23 188 12 105 3.6 1 3 102 13 36 0.9 1 0 36 14 27 0.9 1 0 27 15 33 0.9 1 0 33 16 112 0.9 1 0 112 17 273 0.9 1 0 273 18 148 0.9 1 0 148 19 21 0.9 1 0 21 20 5 0.9 1 0 5 21 1 0.9 1 0 1 22 3 0.9 1 0 3 23 2 0.9 1 0 2 24 1 0.9 1 0 1 25 5 0.9 1 0 5 26 2 0.9 1 0 2 27 3 0.9 1 0 3 28 1 0.9 1 0 1 29 1 0.9 1 0 1 30 1 0.9 1 0 1 31 1 0.9 1 0 1 32 1 0.9 1 0 1 34 2 0.9 1 0 2 36 1 0.9 1 0 1