This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_10_s456.fastq.gz zr1394_10_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 2125.62 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 79,264,583 Reads with adapters: 521,343 (0.7%) Reads written (passing filters): 78,708,932 (99.3%) Total basepairs processed: 3,978,999,570 bp Quality-trimmed: 5,235,908 bp (0.1%) Total written (filtered): 3,956,330,567 bp (99.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 521343 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.8% C: 1.4% G: 34.5% T: 37.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 417959 1238509.1 0 417959 4 81634 309627.3 0 81634 5 12134 77406.8 0 12134 6 1255 19351.7 0 1255 7 4142 4837.9 0 4142 8 1302 1209.5 0 1302 9 671 302.4 0 46 625 10 1592 75.6 1 5 1587 11 253 18.9 1 16 237 12 147 4.7 1 0 147 13 36 1.2 1 0 36 14 23 1.2 1 0 23 15 10 1.2 1 0 10 16 27 1.2 1 0 27 17 77 1.2 1 0 77 18 36 1.2 1 0 36 19 11 1.2 1 0 11 20 3 1.2 1 0 3 22 1 1.2 1 0 1 23 3 1.2 1 0 3 25 1 1.2 1 0 1 26 2 1.2 1 0 2 27 5 1.2 1 0 5 28 4 1.2 1 0 4 29 11 1.2 1 0 11 30 1 1.2 1 0 1 31 2 1.2 1 0 2 33 1 1.2 1 0 1