This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -q 20 -m 36 -O 3 -o fastq/zr1394_11_s456.fastq.gz zr1394_11_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1244.94 s (23 us/read; 2.66 M reads/minute). === Summary === Total reads processed: 55,109,528 Reads with adapters: 284,976 (0.5%) Reads written (passing filters): 54,751,154 (99.3%) Total basepairs processed: 2,769,826,173 bp Quality-trimmed: 3,100,652 bp (0.1%) Total written (filtered): 2,755,623,400 bp (99.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 284976 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.4% C: 2.0% G: 33.6% T: 37.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 226149 861086.4 0 226149 4 45757 215271.6 0 45757 5 7219 53817.9 0 7219 6 683 13454.5 0 683 7 1872 3363.6 0 1872 8 730 840.9 0 730 9 346 210.2 0 39 307 10 807 52.6 1 7 800 11 265 13.1 1 28 237 12 147 3.3 1 0 147 13 59 0.8 1 0 59 14 45 0.8 1 0 45 15 67 0.8 1 0 67 16 182 0.8 1 0 182 17 371 0.8 1 0 371 18 222 0.8 1 0 222 19 24 0.8 1 0 24 20 3 0.8 1 0 3 21 3 0.8 1 0 3 22 2 0.8 1 0 2 23 3 0.8 1 0 3 24 1 0.8 1 0 1 25 3 0.8 1 0 3 26 2 0.8 1 0 2 27 3 0.8 1 0 3 28 3 0.8 1 0 3 29 2 0.8 1 0 2 30 1 0.8 1 0 1 31 1 0.8 1 0 1 34 1 0.8 1 0 1 36 1 0.8 1 0 1 37 1 0.8 1 0 1 38 1 0.8 1 0 1