FastQCFastQC Report
Thu 7 Jul 2022
SRR3321270.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3321270.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4971068
Sequences flagged as poor quality0
Sequence length36
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT435910.8768940597875547TruSeq Adapter, Index 1 (100% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATT322480.6487137170523518TruSeq Adapter, Index 8 (97% over 36bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCC60780.12226748859601197Illumina Single End PCR Primer 1 (100% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)