FastQCFastQC Report
Thu 7 Jul 2022
SRR3321269.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3321269.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9685269
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT1115331.1515735907799773TruSeq Adapter, Index 8 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG540750.5583221281721757TruSeq Adapter, Index 2 (97% over 35bp)
CCACAACATCCTTGGAAAAAGGATCCTGCCATGTTC130170.13439998414086382No Hit
GCCAAATCCATCCCCTTTATGGACAAGCCACTGGGA125580.12966082821241207No Hit
CAACATCCTTGGAAAAAGGATCCTGCCATGTTCTCC123790.12781266064990038No Hit
CCCACATCCGACCAGCTGCATCATGGCAGTTATCAA115560.11931521984572654No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)