FastQCFastQC Report
Thu 7 Jul 2022
SRR3321267.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3321267.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5324659
Sequences flagged as poor quality0
Sequence length36
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACT1273822.392303432013205TruSeq Adapter, Index 8 (100% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC445240.8361850026452398TruSeq Adapter, Index 5 (100% over 35bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCC109510.20566575249231922Illumina Single End PCR Primer 1 (100% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)