FastQCFastQC Report
Thu 7 Jul 2022
SRR3321260.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3321260.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7191423
Sequences flagged as poor quality0
Sequence length36
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT797391.1088069774229663TruSeq Adapter, Index 1 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA491560.6835364850600499TruSeq Adapter, Index 3 (100% over 36bp)
GCCCGGGATAGCTTGCCTCTTTTGGCCGGTGAGTAG176750.24577889522004198No Hit
CTCAGACAGGCGTAGCCCCAGGAGGAACCTGGAGCC151750.21101526081833874No Hit
CGGACACGGAAAGGATTGACAGATTGATAGCTCTTT137730.19151981464586354No Hit
CCTCAGACAGGCGTAGCCCCAGGAGGAACCTGGAGC116980.16266599809244983No Hit
CCCGGGATAGCTTGCCTCTTTTGGCCGGTGAGTAGA97670.13581456688057425No Hit
CCGGACACGGAAAGGATTGACAGATTGATAGCTCTT77170.10730838667117759No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)