FastQCFastQC Report
Thu 7 Jul 2022
SRR3321256.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3321256.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4553909
Sequences flagged as poor quality0
Sequence length36
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG937822.0593736062797916TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA412310.905397977869123TruSeq Adapter, Index 7 (97% over 35bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCC147220.323282700642459Illumina Single End PCR Primer 1 (100% over 36bp)
CCACAACATCCTTGGAAAAAGGATCCTGCCATGTTC55400.12165372650178122No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)