FastQCFastQC Report
Thu 7 Jul 2022
SRR3321250.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3321250.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586364
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG243151.5327503649855898TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA121690.7671001106933844TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGG23060.14536386352690808TruSeq Adapter, Index 19 (97% over 36bp)
CCACAACATCCTTGGAAAAAGGATCCTGCCATGTTC21610.1362234644759967No Hit
GCCAAATCCATCCCCTTTATGGACAAGCCACTGGGA18040.1137191716403045No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG16590.10457877258939309TruSeq Adapter, Index 2 (97% over 35bp)
CAACATCCTTGGAAAAAGGATCCTGCCATGTTCTCC16510.10407447471072212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)