FastQCFastQC Report
Thu 7 Jul 2022
SRR3321247.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3321247.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4663651
Sequences flagged as poor quality0
Sequence length36
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT1739113.729074066648641TruSeq Adapter, Index 9 (100% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA653181.4005765011146845TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGT113490.24335011346260688TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG72520.15550048663589966TruSeq Adapter, Index 2 (97% over 35bp)
CCACAACATCCTTGGAAAAAGGATCCTGCCATGTTC61510.1318923735931355No Hit
CAACATCCTTGGAAAAAGGATCCTGCCATGTTCTCC48790.10461760539114098No Hit
GCCAAATCCATCCCCTTTATGGACAAGCCACTGGGA47760.10240903532447003No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAT46950.10067219867009773TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)