FastQCFastQC Report
Thu 7 Jul 2022
SRR3321244.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3321244.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5487378
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACT1123252.0469703381104782TruSeq Adapter, Index 8 (100% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC539460.983092471486382TruSeq Adapter, Index 5 (100% over 35bp)
CCACAACATCCTTGGAAAAAGGATCCTGCCATGTTC69160.12603469270751896No Hit
CTCCCACATCCGACCAGCTGCATCATGGCAGTTATC60470.11019834974007622No Hit
GCCAAATCCATCCCCTTTATGGACAAGCCACTGGGA56610.103164024785608No Hit
CAACATCCTTGGAAAAAGGATCCTGCCATGTTCTCC56120.10227106643646564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)