FastQCFastQC Report
Thu 7 Jul 2022
SRR3321243.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3321243.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3460768
Sequences flagged as poor quality0
Sequence length36
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG241050.6965216969181407TruSeq Adapter, Index 7 (100% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA78130.22575913785610593TruSeq Adapter, Index 7 (100% over 35bp)
CCACAACATCCTTGGAAAAAGGATCCTGCCATGTTC43080.12448104004660236No Hit
CAACATCCTTGGAAAAAGGATCCTGCCATGTTCTCC38720.11188268037614771No Hit
CTCCCACATCCGACCAGCTGCATCATGGCAGTTATC35850.10358972343710991No Hit

[OK]Adapter Content

Adapter graph