FastQCFastQC Report
Thu 7 Jul 2022
SRR3321242.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3321242.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5365072
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT745011.3886300127938638TruSeq Adapter, Index 8 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG277640.5174953849640788TruSeq Adapter, Index 2 (97% over 35bp)
GCCAAATCCATCCCCTTTATGGACAAGCCACTGGGA57250.10670872636937584No Hit
CCACAACATCCTTGGAAAAAGGATCCTGCCATGTTC55820.10404333809499668No Hit
CAACATCCTTGGAAAAAGGATCCTGCCATGTTCTCC55530.10350280480858412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)