FastQCFastQC Report
Thu 7 Jul 2022
SRR3321235.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3321235.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3921521
Sequences flagged as poor quality0
Sequence length36
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG1916844.888001364776575TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA999832.5495974648612107TruSeq Adapter, Index 2 (97% over 36bp)
CCACAACATCCTTGGAAAAAGGATCCTGCCATGTTC45400.11577140604372639No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)