FastQCFastQC Report
Thu 7 Jul 2022
SRR3321233.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3321233.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4242627
Sequences flagged as poor quality0
Sequence length36
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT454851.0720951900791655TruSeq Adapter, Index 1 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA352980.8319845227968427TruSeq Adapter, Index 3 (100% over 36bp)
GCCCGGGATAGCTTGCCTCTTTTGGCCGGTGAGTAG92340.21764816940070386No Hit
CTCAGACAGGCGTAGCCCCAGGAGGAACCTGGAGCC80750.19033018929073897No Hit
CGGACACGGAAAGGATTGACAGATTGATAGCTCTTT65690.15483331435924017No Hit
CTGACTTTCAATAGATCGCAGCGAGTGAGCTGCTCT62440.1471729661834519No Hit
CCGGGATAGCTTGCCTCTTTTGGCCGGTGAGTAGAG60350.14224677304886807No Hit
CCTCAGACAGGCGTAGCCCCAGGAGGAACCTGGAGC59490.1402197270700441No Hit
CCCGGGATAGCTTGCCTCTTTTGGCCGGTGAGTAGA55770.13145157469652646No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTG53850.12692607669729156No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)