FastQCFastQC Report
Thu 7 Jul 2022
SRR3321225.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3321225.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212595
Sequences flagged as poor quality0
Sequence length36
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACT541812.448753612839223TruSeq Adapter, Index 8 (100% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC176780.7989713436033256TruSeq Adapter, Index 5 (100% over 35bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCC36510.1650098639832414Illumina Single End PCR Primer 1 (100% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)