FastQCFastQC Report
Thu 7 Jul 2022
SRR3321216.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3321216.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9974701
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT1389991.3935154547489694TruSeq Adapter, Index 8 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG558320.55973607629943TruSeq Adapter, Index 2 (97% over 35bp)
CCACAACATCCTTGGAAAAAGGATCCTGCCATGTTC118860.11916146659433702No Hit
CAACATCCTTGGAAAAAGGATCCTGCCATGTTCTCC117190.11748723094556919No Hit
GCCAAATCCATCCCCTTTATGGACAAGCCACTGGGA114770.1150610930593308No Hit
CCCACATCCGACCAGCTGCATCATGGCAGTTATCAA106080.10634905246783839No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)