SUMMARISING RUN PARAMETERS ========================== Input filename: heart_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.7 Cutadapt version: 3.4 Python version: could not detect Number of cores used for trimming: 4 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 2296). Second best hit was Nextera (count: 8) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) 2-colour high quality G-trimming enabled, with quality cutoff: --nextseq-trim=20 Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Output file will be GZIP compressed This is cutadapt 3.4 with Python 3.9.6 Command line parameters: -j 4 -e 0.1 --nextseq-trim=20 -O 1 -a AGATCGGAAGAGC heart_1.fastq.gz Processing reads on 4 cores in single-end mode ... Finished in 1091.44 s (10 µs/read; 5.76 M reads/minute). === Summary === Total reads processed: 104,706,949 Reads with adapters: 26,435,890 (25.2%) Reads written (passing filters): 104,706,949 (100.0%) Total basepairs processed: 14,971,459,792 bp Quality-trimmed: 381,516,818 bp (2.5%) Total written (filtered): 14,506,392,659 bp (96.9%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 26435890 times No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.8% C: 29.9% G: 12.1% T: 20.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 23849093 26176737.2 0 23849093 3 1232926 1636046.1 0 1232926 4 357554 409011.5 0 357554 5 136091 102252.9 0 136091 8 10004 1597.7 0 10004 9 4676 399.4 0 4676 10 1160 99.9 1 0 1160 11 7792 25.0 1 2420 5372 12 2818 6.2 1 0 2818 13 9430 1.6 1 1554 7876 14 12272 1.6 1 1496 10776 15 11858 1.6 1 1604 10254 16 15724 1.6 1 3854 11870 17 18931 1.6 1 3377 15554 18 9537 1.6 1 4085 5452 19 6013 1.6 1 1684 4329 20 12202 1.6 1 4227 7975 21 9707 1.6 1 3321 6386 22 11905 1.6 1 6292 5613 23 3183 1.6 1 1078 2105 24 9423 1.6 1 4480 4943 25 8527 1.6 1 3083 5444 26 11085 1.6 1 5891 5194 27 15866 1.6 1 7417 8449 28 15199 1.6 1 5830 9369 29 14217 1.6 1 8589 5628 30 5387 1.6 1 3488 1899 31 7527 1.6 1 4124 3403 32 7838 1.6 1 4568 3270 33 17962 1.6 1 11477 6485 34 6827 1.6 1 2745 4082 35 13587 1.6 1 9123 4464 36 5252 1.6 1 3618 1634 37 6588 1.6 1 3870 2718 38 7621 1.6 1 4298 3323 39 9443 1.6 1 6373 3070 40 9743 1.6 1 6176 3567 41 5882 1.6 1 3172 2710 42 11390 1.6 1 7396 3994 43 5740 1.6 1 3558 2182 44 7423 1.6 1 5007 2416 45 2868 1.6 1 1828 1040 46 6092 1.6 1 3792 2300 47 2592 1.6 1 1526 1066 48 8031 1.6 1 5412 2619 49 1352 1.6 1 799 553 50 6904 1.6 1 4637 2267 51 1475 1.6 1 924 551 52 5759 1.6 1 4029 1730 53 4274 1.6 1 2460 1814 54 2867 1.6 1 1822 1045 55 5658 1.6 1 4069 1589 56 1397 1.6 1 841 556 57 2200 1.6 1 1443 757 58 2420 1.6 1 1630 790 59 2413 1.6 1 1586 827 60 2733 1.6 1 1779 954 61 1559 1.6 1 1087 472 62 2985 1.6 1 2018 967 63 1321 1.6 1 797 524 64 3546 1.6 1 2209 1337 65 1415 1.6 1 976 439 66 3317 1.6 1 2269 1048 67 785 1.6 1 447 338 68 1556 1.6 1 961 595 69 1333 1.6 1 819 514 70 1431 1.6 1 830 601 71 1465 1.6 1 824 641 72 1572 1.6 1 743 829 73 1793 1.6 1 783 1010 74 2765 1.6 1 864 1901 75 12394 1.6 1 1073 11321 76 21620 1.6 1 1191 20429 77 46344 1.6 1 1339 45005 78 56023 1.6 1 1243 54780 79 45345 1.6 1 790 44555 80 33414 1.6 1 475 32939 81 23487 1.6 1 297 23190 82 17330 1.6 1 209 17121 83 12790 1.6 1 157 12633 84 9833 1.6 1 134 9699 85 9863 1.6 1 104 9759 86 8118 1.6 1 93 8025 87 6590 1.6 1 100 6490 88 5799 1.6 1 98 5701 89 4885 1.6 1 84 4801 90 3731 1.6 1 78 3653 91 3112 1.6 1 51 3061 92 2631 1.6 1 48 2583 93 2169 1.6 1 46 2123 94 1686 1.6 1 41 1645 95 1518 1.6 1 44 1474 96 1262 1.6 1 37 1225 97 1161 1.6 1 31 1130 98 996 1.6 1 24 972 99 938 1.6 1 26 912 100 774 1.6 1 23 751 101 677 1.6 1 21 656 102 639 1.6 1 22 617 103 660 1.6 1 24 636 104 661 1.6 1 20 641 105 686 1.6 1 21 665 106 621 1.6 1 8 613 107 741 1.6 1 12 729 108 690 1.6 1 12 678 109 655 1.6 1 18 637 110 635 1.6 1 15 620 111 610 1.6 1 20 590 112 586 1.6 1 9 577 113 587 1.6 1 21 566 114 511 1.6 1 20 491 115 464 1.6 1 14 450 116 430 1.6 1 14 416 117 415 1.6 1 17 398 118 360 1.6 1 16 344 119 349 1.6 1 14 335 120 319 1.6 1 17 302 121 295 1.6 1 13 282 122 285 1.6 1 13 272 123 273 1.6 1 16 257 124 274 1.6 1 10 264 125 229 1.6 1 12 217 126 235 1.6 1 13 222 127 236 1.6 1 14 222 128 188 1.6 1 15 173 129 213 1.6 1 13 200 130 222 1.6 1 10 212 131 160 1.6 1 15 145 132 176 1.6 1 16 160 133 206 1.6 1 21 185 134 168 1.6 1 21 147 135 181 1.6 1 12 169 136 191 1.6 1 19 172 137 173 1.6 1 12 161 138 189 1.6 1 24 165 139 205 1.6 1 14 191 140 214 1.6 1 12 202 141 210 1.6 1 12 198 142 225 1.6 1 17 208 143 278 1.6 1 8 270 144 339 1.6 1 10 329 145 461 1.6 1 11 450 146 715 1.6 1 9 706 147 1565 1.6 1 9 1556 148 4133 1.6 1 22 4111 149 7800 1.6 1 7 7793 150 37381 1.6 1 1 37380 151 22080 1.6 1 1 22079 RUN STATISTICS FOR INPUT FILE: heart_1.fastq.gz ============================================= 104706949 sequences processed in total