FastQCFastQC Report
Thu 24 Mar 2022
gonad_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamegonad_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69648408
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG24948563.582071825676188Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT8338441.197219037655534Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1951440.2801844372379624No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN824490.11837887234981738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)