FastQCFastQC Report
Thu 24 Mar 2022
gonad_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamegonad_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69648408
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC28329064.067438267935715TruSeq Adapter, Index 6 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC7883891.131955521510269TruSeq Adapter, Index 6 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT1995260.28647603833241964TruSeq Adapter, Index 6 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1767220.25373444286048863No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN805410.11563939839084333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)