FastQCFastQC Report
Tue 29 Jun 2021
S9M_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS9M_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37607822
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTGTAGA5105401.357536737969032Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTGT3823431.0166581835023576Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTGT3326450.8845101425974629Illumina Single End PCR Primer 1 (97% over 34bp)
GGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTGT2689960.7152660954415281Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTGTA1992170.5297222476749651Illumina Single End PCR Primer 1 (97% over 34bp)
GGAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTGT1560510.4149429339460286Illumina Single End PCR Primer 1 (97% over 34bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTGTAGA1443500.38382972563526807Illumina Single End PCR Primer 1 (97% over 34bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTGTAG929940.24727302740371404Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTG856840.22783558165107246Illumina Single End PCR Primer 1 (97% over 34bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTGTAGATC856320.22769731254311934Illumina Single End PCR Primer 1 (97% over 34bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTGTAGA852240.22661243184994867Illumina Single End PCR Primer 1 (97% over 34bp)
GGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTGTA818930.2177552318770281Illumina Single End PCR Primer 1 (97% over 34bp)
GGAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTGT728950.1938293581585235Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTG707880.18822680026511507Illumina Single End PCR Primer 1 (97% over 34bp)
GGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTGTA560050.148918488286825Illumina Single End PCR Primer 1 (97% over 34bp)
GGAGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTG505690.1344640484631096Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTG486990.1294916786194106Illumina Single End PCR Primer 1 (97% over 34bp)
GAGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTGT464710.12356737914788046Illumina Single End PCR Primer 1 (97% over 34bp)
GAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTGT422220.11226919761532587Illumina Single End PCR Primer 1 (97% over 34bp)
GGGAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTG414490.1102137741451765Illumina Single End PCR Primer 1 (97% over 34bp)
AGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTGTAGGTGT384710.10229520869355316Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)