FastQCFastQC Report
Tue 29 Jun 2021
S9M_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS9M_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37607822
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATC4108981.092586536917772TruSeq Adapter, Index 27 (97% over 38bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATCTCG3790271.0078408688490388TruSeq Adapter, Index 27 (97% over 38bp)
TCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATC3549050.9436999568866286TruSeq Adapter, Index 27 (97% over 38bp)
CTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATC2877320.7650855186455626TruSeq Adapter, Index 27 (97% over 38bp)
CCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATC1680970.44697350460763186TruSeq Adapter, Index 27 (97% over 38bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATCGCG1636760.43521797141030927TruSeq Adapter, Index 27 (97% over 38bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATCT1407380.37422534067513935TruSeq Adapter, Index 27 (97% over 38bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATCTCG1089340.28965782703396115TruSeq Adapter, Index 27 (97% over 38bp)
CTCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAAT923540.24557125376736788TruSeq Adapter, Index 27 (97% over 38bp)
TCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATC782270.20800725976633264TruSeq Adapter, Index 27 (97% over 38bp)
TCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAAT763570.20303488992263366TruSeq Adapter, Index 27 (97% over 38bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATCG724720.19270459214575095TruSeq Adapter, Index 27 (97% over 38bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATCTC670770.17835917219561398TruSeq Adapter, Index 27 (97% over 38bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATCTCG621250.16519169868438538TruSeq Adapter, Index 27 (97% over 38bp)
TCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATCT574890.15286447590610272TruSeq Adapter, Index 27 (97% over 38bp)
CCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAAT542400.14422531568033906TruSeq Adapter, Index 27 (97% over 38bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAAT524260.13940185102982033TruSeq Adapter, Index 27 (97% over 38bp)
CCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATC496710.13207624732961137TruSeq Adapter, Index 27 (97% over 38bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATCGCG458890.12201982874732815TruSeq Adapter, Index 27 (97% over 38bp)
CTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATC453870.1206850000513191TruSeq Adapter, Index 27 (97% over 38bp)
CCTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAAT442060.11754469588799905TruSeq Adapter, Index 27 (97% over 38bp)
CCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATC416130.1106498536394902TruSeq Adapter, Index 27 (97% over 38bp)
CTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAAT401200.10667993482845138TruSeq Adapter, Index 27 (97% over 38bp)
CTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGGAATCT400650.10653368865657788TruSeq Adapter, Index 27 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)