FastQCFastQC Report
Tue 29 Jun 2021
S7M_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS7M_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29392763
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTGTAGA3006661.0229252690534743Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTGT1874640.6377896491051216Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTGT1859880.6327680048316655Illumina Single End PCR Primer 1 (97% over 35bp)
GGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTGT1586830.5398709879707464Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTGTA1077600.36662085833849645Illumina Single End PCR Primer 1 (97% over 35bp)
GGAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTGT864330.29406218122467764Illumina Single End PCR Primer 1 (97% over 35bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTGTAGA799210.27190706773636764Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTGTAGATC653540.22234724921913604Illumina Single End PCR Primer 1 (97% over 35bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTGTAG526040.17896922449924152Illumina Single End PCR Primer 1 (97% over 35bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTGTAGA489010.16637088524137728Illumina Single End PCR Primer 1 (97% over 35bp)
GGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTGTA483280.16442142577749497Illumina Single End PCR Primer 1 (97% over 35bp)
GGAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTGT451250.15352418552825403Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTG435330.14810788628479737Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTG371620.12643248271691912Illumina Single End PCR Primer 1 (97% over 35bp)
GGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTGTA350860.11936951963311512Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)