FastQCFastQC Report
Tue 29 Jun 2021
S7M_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS7M_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29392763
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATCTCG2130110.7247056018517212TruSeq Adapter, Index 11 (97% over 37bp)
TCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATC1886980.6419879614584039TruSeq Adapter, Index 11 (97% over 37bp)
CCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATC1875130.6379563568079666TruSeq Adapter, Index 11 (97% over 37bp)
CTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATC1600080.5443789003435981TruSeq Adapter, Index 11 (97% over 37bp)
CCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATC867630.295184906570369TruSeq Adapter, Index 11 (97% over 37bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATCGCG865000.29429012849183317TruSeq Adapter, Index 11 (97% over 37bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATCT739480.25158573897935355TruSeq Adapter, Index 11 (97% over 37bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATCTCG570840.19421107161650641TruSeq Adapter, Index 11 (97% over 37bp)
TCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATC456190.15520487134877384TruSeq Adapter, Index 11 (97% over 37bp)
CTCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGAT440660.14992125782798985TruSeq Adapter, Index 11 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATCTCGTA434220.14773024230488302TruSeq Adapter, Index 11 (97% over 37bp)
TCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGAT376370.12804852677511128TruSeq Adapter, Index 11 (97% over 37bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATCTC364960.12416661883743287TruSeq Adapter, Index 11 (97% over 37bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATCG346360.11783853052535415TruSeq Adapter, Index 11 (97% over 37bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATCTCG340980.11600814799207547TruSeq Adapter, Index 11 (97% over 37bp)
TCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATCT333390.11342587969698527TruSeq Adapter, Index 11 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)