FastQCFastQC Report
Tue 29 Jun 2021
S77F_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS77F_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27897791
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTA715350.2564181515303488No Hit
CCTACCTAACCATATCCTTAGCAATATTAAGGCTGCACTTAAAAGCATTT651420.23350235866345118No Hit
CACCATGTTACGACTTACCTCTCCTTTCGGGTAGGGTGACGGGCGATTTG545850.19566065284523781No Hit
CGCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAG537770.19276436618225434No Hit
CCACCGTATGCTTTACATTTTAAATCACTGATTAACTGCCTCTAAGCTGC452700.16227091241740252No Hit
TCACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATT448120.16062920537328565No Hit
CTCCTTTACATAATCGGCTTCAACACATTATGAATTTATTCTGCATTGTG437610.1568618820034891No Hit
CCATCGGATTAAGCGCCAAGAGCCTCTGGAGGGTTCTACGGAACCGCCAA420700.15080047018776505No Hit
CTACGGAACCGCCAAGTTCTTCGAGTTTTAAGCAGTTGCTCGTAGTACTC420280.15064992063350105No Hit
CTTCGACCTGACGTAGCCTCAGTACTTTAAGTTTTTAAAATACTAAGAAC378050.13551252140357636No Hit
ACCATGTTACGACTTACCTCTCCTTTCGGGTAGGGTGACGGGCGATTTGA363320.13023253346474636No Hit
CTGCCCACCTTTTAGGTTACTGACTAGCTCCTTTACATAATCGGCTTCAA349210.1251747853441156No Hit
CCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTAAA344400.12345063449647321No Hit
CTCCCAAATCCTCTTTGCTTAGAGAGTTTCATCTCCATGCTTAGTATGTC317610.1138477236423486No Hit
CTCCTAACAAACCACCACCGGTAACTTCGATCACCGGAACGTAGCCATAC301710.10814834765949749No Hit
CTCCTGCGGGGCTTGAATTTTATCTCGATTCTCCCACCGTATGCTTTACA297980.10681132423710536No Hit
CTCAGCTAACCCCTTGCCCATAAGGAACACTTCGACCTGACGTAGCCTCA286940.10285402166788044No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)