FastQCFastQC Report
Tue 29 Jun 2021
S76F_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS76F_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26071715
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACCTAACCATATCCTTAGCAATATTAAGGCTGCGCTTAAAAGCATTT874560.3354439859441544No Hit
CACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTA656810.2519243555707785No Hit
CGCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAG611440.2345223549735796No Hit
CCACCGTATGCTTTACATTTTAAATCACTGATTAACTGCCTCTAAGCTGC553240.21219931255001828No Hit
TCACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATT443510.17011155576071615No Hit
CACCATGTTACGACTTACCTCTCCTTTCGGGTAGGGTGACGGGCGATTTG431800.16562009825590684No Hit
CTACGGAACCGCCAAGTTCTTCGAGTTTTAAGCAGTTGCTCGTAGTACTC389460.14938027667148096No Hit
CTTCGACCTGACGTAGCCTCAGTACTTTAAGTTTTTAAAATACTAAGAAC382530.14672222368187132No Hit
CTGCCCACCTTTTAGGTTACTGACTAGCTCCTTTACATAATCGGCTTCAA359620.13793492296153131No Hit
CTCCTTTACATAATCGGCTTCAACACATTATGAATTTATTCTGCATTGTG355420.13632398175570729No Hit
CCATCGGATTAAGCGCCAAGAGCCTCTGGAGGGTTCTACGGAACCGCCAA332590.1275673656297639No Hit
CTCCCAAATCCTCTTTGCTTAGAGAGTTTCATCTCCATGCTTAGTATGTC313570.12027210331196088No Hit
CCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTAAA303810.11652858279556984No Hit
ACCATGTTACGACTTACCTCTCCTTTCGGGTAGGGTGACGGGCGATTTGA286540.10990454598019347No Hit
CCACCGGTAACTTCGATCATCGGAACGTAGCCATACTTGACATTATGGAA268810.1031040727470364No Hit
CAGGCCCACAATCAAAAGGCAAGGAATTTCGCTACCTTAGCACCCTCACG268290.10290462288345817No Hit
CTCAGCTAACCCCTTGCCCATAAGGAACACTTCGACCTGACGTAGCCTCA267970.1027818845058716No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)