FastQCFastQC Report
Tue 29 Jun 2021
S6M_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS6M_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43766517
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGTAGA7301531.6682913104554336Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGT5470721.2499783795909554Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGT4957471.1327083669920546Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGT3780130.8637036390170139Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGTA2946780.6732955240646634Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGT2487830.5684322561011651Illumina Single End PCR Primer 1 (96% over 33bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGTAGA2425100.5540993814975042Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGTAGA2286780.5224953130266226Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGTA1428110.32630195361444914Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGTAG1374340.31401630611821363Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGT1256830.28716701399839517Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTG1139850.26043881901774363Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGTAGATC1037610.23707849541694165Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTG936940.21407689353027565Illumina Single End PCR Primer 1 (96% over 33bp)
GGGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGT865120.19766708874731795Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGTA805020.18393513013612667Illumina Single End PCR Primer 1 (96% over 33bp)
GAGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGT735210.16798458054133025Illumina Single End PCR Primer 1 (96% over 33bp)
GAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGT734660.16785891369879855Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTG696150.15905994986989713Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTG689940.1576410569751301Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGT683670.1562084549702687Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTG573300.13099054695168Illumina Single End PCR Primer 1 (96% over 33bp)
GAGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGT560130.12798139728596636Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGT557670.1274193237720973Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTG549840.12563028490478234Illumina Single End PCR Primer 1 (96% over 33bp)
AGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGT547090.12500195069212386Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTG536570.12259828672224478Illumina Single End PCR Primer 1 (96% over 33bp)
AGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGTA439500.1004192314412408Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)