FastQCFastQC Report
Tue 29 Jun 2021
S6M_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS6M_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43766517
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATC5543851.2666874999443067TruSeq Adapter, Index 3 (97% over 37bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCTCG5097061.1646026116266002TruSeq Adapter, Index 3 (97% over 37bp)
TCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATC4996691.1416695552904061TruSeq Adapter, Index 3 (97% over 37bp)
CTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATC3830220.8751484610941282TruSeq Adapter, Index 3 (97% over 37bp)
CCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATC2515250.574697319414291TruSeq Adapter, Index 3 (97% over 37bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCGCG2271210.5189377989571342TruSeq Adapter, Index 3 (97% over 37bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCT1985840.4537349864966408TruSeq Adapter, Index 3 (97% over 37bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCTCG1706640.3899419275241847TruSeq Adapter, Index 3 (97% over 37bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCTCG1566520.3579265857504722TruSeq Adapter, Index 3 (97% over 37bp)
TCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATC1273300.290930164719299TruSeq Adapter, Index 3 (97% over 37bp)
CTCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCAT1158290.2646520855200792TruSeq Adapter, Index 3 (97% over 37bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCG1000550.22861083508198743TruSeq Adapter, Index 3 (97% over 37bp)
TCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCT966120.220744090739503TruSeq Adapter, Index 3 (97% over 37bp)
TCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCAT949680.21698779457364634TruSeq Adapter, Index 3 (97% over 37bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCTC938150.2143533605838454TruSeq Adapter, Index 3 (97% over 37bp)
ATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATC886330.2025132591656768TruSeq Adapter, Index 3 (97% over 37bp)
CTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATC745410.17031512925737272TruSeq Adapter, Index 3 (97% over 37bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATC743150.16979875277715156TruSeq Adapter, Index 3 (97% over 37bp)
CCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATC742000.16953599483367615TruSeq Adapter, Index 3 (97% over 37bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCGCG741030.16931436422048388TruSeq Adapter, Index 3 (97% over 37bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCGCG731900.16722829463445768TruSeq Adapter, Index 3 (97% over 37bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCAT704210.16090154032590714TruSeq Adapter, Index 3 (97% over 37bp)
CCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCAT695840.15898911946774288TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCTCGTA634940.14507437272196003TruSeq Adapter, Index 3 (97% over 37bp)
CCTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCAT579400.13238430647794067TruSeq Adapter, Index 3 (97% over 37bp)
TCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATC562700.12856860416834173TruSeq Adapter, Index 3 (97% over 37bp)
CTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCAT557870.12746502080574518TruSeq Adapter, Index 3 (97% over 37bp)
TTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATC556750.127209117417317TruSeq Adapter, Index 3 (97% over 37bp)
CTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCT552200.12616950990182746TruSeq Adapter, Index 3 (97% over 37bp)
CCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATC550720.1258313518528331TruSeq Adapter, Index 3 (97% over 37bp)
ATCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCAT550140.1256988304552542TruSeq Adapter, Index 3 (97% over 37bp)
TCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCG471150.10765078701601959TruSeq Adapter, Index 3 (97% over 37bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCGC451110.10307194424450089TruSeq Adapter, Index 3 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)