FastQCFastQC Report
Tue 29 Jun 2021
S64M_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS64M_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23911361
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAATCATTACCTCGTGTTCCGAAAACCAACAAAATAGAACCGAGGTCC1752300.7328315606961896No Hit
CCGAAAACCAACAAAATAGAACCGAGGTCCTATTCCATTATTCCATGCAC1387000.5800589937143269No Hit
CCACCCGGGCTCACGCCCGAAGCTTCAAGGCTCACCGCGACGGCCCTCTT512340.21426634811795112No Hit
CTCTTAATCATTACCTCGTGTTCCGAAAACCAACAAAATAGAACCGAGGT478070.19993424882841257No Hit
CCCTCTTAATCATTACCTCGTGTTCCGAAAACCAACAAAATAGAACCGAG460470.1925737309557578No Hit
CTAAGAAGTTGCACCGACAGCATAGATCGGTGAACTATTTAGTAGGTTAG410110.17151261277013885No Hit
CCATGCACCATTGTGCAGGCGAATCGCCTGCTTTGAACACTCTAATTTTT404870.169321185858053No Hit
CTCTCGTACTGAGCAGGATTACCGTTGCAACGACTGCAGTCATCAGTAGG393290.16447829966684038No Hit
TCCCTCTTAATCATTACCTCGTGTTCCGAAAACCAACAAAATAGAACCGA386180.16150481773078496No Hit
CGTGTTCCGAAAACCAACAAAATAGAACCGAGGTCCTATTCCATTATTCC380320.15905409984818514No Hit
ACCTCGTGTTCCGAAAACCAACAAAATAGAACCGAGGTCCTATTCCATTA343370.1436011944280378No Hit
CCCTAGCACGAAGGAGGTTCAACGGGTTACCCGACCCTTTCGGGCCAGGA342370.1431829831852733No Hit
CCTACCTAACCATATCCTTAGCAATATTAAGGCTGCGCTTAAAAGCATTT339060.1417987039717229No Hit
CAATCAAGAGCACCAAGGGTTTACAACCGAGAGGTTGGGTCAGAACAGGC318830.13333829053059756No Hit
CAACGGGTTACCCGACCCTTTCGGGCCAGGACAGATACCTGTTGATTCCT299180.12512043961027564No Hit
CTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTGCACCGACAGCA282150.11799830214599663No Hit
CTTTCGGGCCAGGACAGATACCTGTTGATTCCTTCAGTGTAGCGCGCGTG270910.11329760777732392No Hit
CACCAAAACGGACGGCCTTGCGACCGATTGGTTTTGATCTAATAAAAGCG265140.11088452890657291No Hit
CCCTCTAAGAAGTTGCACCGACAGCATAGATCGGTGAACTATTTAGTAGG241280.10090600865421252No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)