FastQCFastQC Report
Tue 29 Jun 2021
S54F_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS54F_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23173114
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACCTAACCATATCCTTAGCAATATTAAGGCTGCACTTAAAAGCATTT336960.14540989182550088No Hit
CACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTA330630.14267827793882168No Hit
CGCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAG319760.13798749706232835No Hit
CACCATGTTACGACTTACCTCTCCTTTCGGGTAGGGTGACGGGCGATTTG308390.1330809488962079No Hit
CTCCTTTACATAATCGGCTTCAACACATTATGAATTTATTCTGCATTGTG303410.1309319066915219No Hit
CCTTTACATAATCGGCTTCAACACATTATGAATTTATTCTGCATTGTGTA303030.13076792355140532No Hit
CCCGCCGCTACAACTGCCCACCTTTTAGGTTACTGACTAGCTCCTTTACA267860.11559085239903451No Hit
TCACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATT267050.11524130938983859No Hit
CCGCCGCTACAACTGCCCACCTTTTAGGTTACTGACTAGCTCCTTTACAT240700.10387037322648997No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)