FastQCFastQC Report
Tue 29 Jun 2021
S53F_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS53F_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23169880
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTAAGCTCTATTA555470.23973797015780832No Hit
CCTACCTAACCATATCCTTAGCAATATTAAGGCTGCACTTAAAAGCATTT421450.1818956334689692No Hit
CACCATGTTACGACTTACCTCTCCTTTCGGGTAGGGTGACGGGCGATTTG389830.1682486055171628No Hit
TCACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTAAGCTCTATT330590.14268092886109035No Hit
CTCCTTTACATAATCGGCTTCAACACATTATGAATTTATTCTGCATTGTG308450.13312541972595454No Hit
CGCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAG271330.1171046203087802No Hit
ACCATGTTACGACTTACCTCTCCTTTCGGGTAGGGTGACGGGCGATTTGA253000.10919348740692658No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)