FastQCFastQC Report
Tue 29 Jun 2021
S50F_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS50F_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20280193
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTA503660.24835069370395044No Hit
CCTACCTAACCATATCCTTAGCAATATTAAGGCTGCACTTAAAAGCATTT447950.22088054092976334No Hit
CCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTAAA340260.1677794683709371No Hit
CACCATGTTACGACTTACCTCTCCTTTCGGGTAGGGTGACGGGCGATTTG285170.14061503260841748No Hit
TCACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATT259270.12784395099198512No Hit
CTGAGGTCGGAGTGATTGGTTAAAAAAGGAGAGAACGCGCTCTCTTTTTT246050.1213252753561073No Hit
CTGCCCACCTTTTAGGTTACTGACTAGCTCCTTTACATAATCGGCTTCAA239960.11832234535440564No Hit
CGCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAG239390.11804128294045327No Hit
CTCCTTTACATAATCGGCTTCAACACATTATGAATTTATTCTGCATTGTG238510.11760736202066716No Hit
CTTCGACCTGACGTAGCCTCAGTACTTTAAGTTTTTAAAATACTAAGAAC237010.11686772408921356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)