FastQCFastQC Report
Tue 29 Jun 2021
S48M_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS48M_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67430692
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGTAGA12385051.8367081269164494Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGT9591101.4223641661574524Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGT8323341.2343548246546245Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGT6288760.9326257544561459Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGTA5071360.7520848221459747Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGT3982430.5905960448989609Illumina Single End PCR Primer 1 (96% over 33bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGTAGA3739990.5546420908745827Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGTAGA2518800.37353909996949164Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGTAG2369880.3514542013004998Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGTA2150490.3189185719760966Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTG2097470.31105568366405023Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGT1802170.2672625693949574Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTG1662080.24648716344183447Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGTAGATC1617410.23986258364366184Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGTA1295620.19214099122696232Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTG1211930.17972972900826822Illumina Single End PCR Primer 1 (96% over 33bp)
GAGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGT1211430.1796555787978566Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTG1183570.1755239290737221Illumina Single End PCR Primer 1 (96% over 33bp)
GAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGT1110190.16464164419371524Illumina Single End PCR Primer 1 (96% over 33bp)
GGGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGT1096710.16264255452101842Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTG1023310.1517573036325951Illumina Single End PCR Primer 1 (96% over 33bp)
AGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGT950120.14090319583254463Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGT907480.13457966588864312Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTG877630.13015289832707042Illumina Single End PCR Primer 1 (96% over 33bp)
GAGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGT849290.12595006440094075Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGT838190.12430392972980316Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTG753700.11177402717445048Illumina Single End PCR Primer 1 (96% over 33bp)
AGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGTA742020.1100418782592354Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)