FastQCFastQC Report
Tue 29 Jun 2021
S48M_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS48M_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67430692
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATCTCG11119901.64908584951197TruSeq Adapter, Index 22 (97% over 38bp)
CCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATC9805211.454116769259909TruSeq Adapter, Index 22 (97% over 38bp)
TCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATC8500841.2606781493507437TruSeq Adapter, Index 22 (97% over 38bp)
CTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATC6434420.954227193753254TruSeq Adapter, Index 22 (97% over 38bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATCT4478910.6642242378292662TruSeq Adapter, Index 22 (97% over 38bp)
CCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATC4063130.6025638888593936TruSeq Adapter, Index 22 (97% over 38bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATCTCG3400500.5042955810093125TruSeq Adapter, Index 22 (97% over 38bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATCTCG2247350.3332829507370323TruSeq Adapter, Index 22 (97% over 38bp)
CTCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTAT2157640.3199789199849825TruSeq Adapter, Index 22 (97% over 38bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATCTC2122030.3146979419994681TruSeq Adapter, Index 22 (97% over 38bp)
TCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATCT1901150.28194134504803836TruSeq Adapter, Index 22 (97% over 38bp)
TCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATC1854960.2750913486102145TruSeq Adapter, Index 22 (97% over 38bp)
TCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTAT1714600.25427590154346924TruSeq Adapter, Index 22 (97% over 38bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATCGCG1417980.21028703071888985TruSeq Adapter, Index 22 (97% over 38bp)
CCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTAT1242220.18422174875500313TruSeq Adapter, Index 22 (97% over 38bp)
CCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATC1234050.18301013431687754TruSeq Adapter, Index 22 (97% over 38bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTAT1213850.18001446581624878TruSeq Adapter, Index 22 (97% over 38bp)
CTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATCT1158020.17173485332168917TruSeq Adapter, Index 22 (97% over 38bp)
CTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATC1138960.16890824730079887TruSeq Adapter, Index 22 (97% over 38bp)
ATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATC1133930.16816229618405815TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATCTCGTA1066380.15814460275745057TruSeq Adapter, Index 22 (97% over 38bp)
CCTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTAT1050370.15577031302007105TruSeq Adapter, Index 22 (97% over 38bp)
CCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATC986670.1463235762136328TruSeq Adapter, Index 22 (97% over 38bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATC985410.1461367176833956TruSeq Adapter, Index 22 (97% over 38bp)
CTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTAT907280.13455000580447848TruSeq Adapter, Index 22 (97% over 38bp)
TCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATC868050.1287321802955841TruSeq Adapter, Index 22 (97% over 38bp)
TTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATC844840.12529012752827748TruSeq Adapter, Index 22 (97% over 38bp)
ATCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTAT784330.11631646906426528TruSeq Adapter, Index 22 (97% over 38bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATCG691390.1025334279529565TruSeq Adapter, Index 22 (97% over 38bp)
TCTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTAT687330.10193132824441427TruSeq Adapter, Index 22 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)